Quenching of nucleotide-derived radicals bybisbenzimidazole derivative Hoechst-33258 in aqueous solution
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چکیده
منابع مشابه
Quantitation of DNA using Hoechst 33258
An essential element of cellular and molecular biology is the ability to quantitate DNA in large numbers of samples at a sensitivity that enables determination of small amounts of sample. Here we describe a fluorescent method to quantitate DNA with Hoechst 33258 dye and the BioTek FL600 fluorescence microplate reader. This assay provides linear results over three orders of magnitude and can rou...
متن کاملBuoyant density of DNA-Hoechst 33258 (bisbenzimide) complexes in CsCl gradients: Hoechst 33258 binds to single AT base pairs.
Buoyant density of DNA in CsCl gradients with Hoechst 33258 (bisbenzimide) was investigated as a function of guanine plus cytosine content of the DNA (%GC; in mole percent). A formula for calculating %GC from the refractive index (nD) of the isopycnic CsCl/Hoechst 33258 solution over the range of 0-75 %GC was established: %GC = 351762.28 X nD - 123778.66 X nD2 - 249789.47 (the coefficients must...
متن کاملVisualization of complexes of Hoechst 33258 and DNA duplexes in solution by atomic force microscopy.
Tertiary structure changes in DNA duplexes, induced by Hoechst 33258 binding, have been examined by the use of atomic force microscopy. Besides minor groove binding, which is an established mode of binding for this drug, Hoechst 33258 has now been found to show another binding mode, which causes an unwinding of the duplex. When the drug concentration is as high as 0.5 microg/ml, the Hoechst 332...
متن کاملDifferent Modes of Binding of Hoechst 33258 with Dna
Spectrophotometric study of Hoechst 33258 binding to DNA has been investigated. It was shown that this ligand forms at least two types of complexes at 0.002 M and 0.02M ionic strengths of solution. One of them is strong, the other is weak. It was revealed that at 0.02M ionic strength of solution, the binding constant value by strong mode is one order higher than at 0.002M. It was obtained that ...
متن کاملSupramolecular assembly of Hoechst-33258 with cucurbit[7]uril macrocycle.
Molecular assemblies of potential guest molecules through non-covalent host-guest interactions have found immense use in many applied areas. In this study supramolecular interaction of a biologically important dye Hoechst-33258 (H33258) has been investigated in aqueous solutions at different pHs, in the presence of a macrocyclic host, namely, cucurbit[7]uril (CB7). The pH dependent emission beh...
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ژورنال
عنوان ژورنال: Journal of Chemical Sciences
سال: 2000
ISSN: 0253-4134,0973-7103
DOI: 10.1007/bf02704354